The following highways are numbered 334:

Australia

• Boort-Mitiamo Rd
• Echuca-Mitiamo Rd

Canada

• Manitoba Provincial Road 334
• Newfoundland and Labrador Route 334
• Nova Scotia Route 334
• Prince Edward Island Route 334
• Saskatchewan Highway 334

Costa Rica

• National Route 334

India

• National Highway 334 (India)

Japan

• Japan National Route 334

United States

• Connecticut Route 334
• Georgia State Route 334
• Indiana State Road 334 (former)
• Maryland Route 334
• New York:
  • New York State Route 334
  • County Route 334 (Erie County, New York)
  • County Route 334 (Wayne County, New York)
• Ohio State Route 334
• Oregon Route 334
• Puerto Rico Highway 334
• Tennessee State Route 334
• Texas:
  • Texas State Highway 334
  • Texas State Highway Loop 334 (former)
• Virginia State Route 334
• Wyoming Highway 334