Route 220 or Highway 220 may refer to:

Australia

• Sunraysia Highway

Canada

• Manitoba Provincial Road 220
• Newfoundland and Labrador Route 220
• Quebec Route 220
• Saskatchewan Highway 220

China

• China National Highway 220 (G220)

Costa Rica

• National Route 220

Japan

• Japan National Route 220

United Kingdom

• A220 road

United States

• Interstate 220
• U.S. Route 220
• Arkansas Highway 220
• California State Route 220
• Georgia State Route 220
• Iowa Highway 220
• Kentucky Route 220
• Maine State Route 220
• Route 220 (Connecticut–Massachusetts)
• Minnesota State Highway 220
• New Mexico State Road 220
• New York State Route 220
• Ohio State Route 220
• Tennessee State Route 220
• Texas State Highway 220
• Utah State Route 220 (former)
• Washington State Route 220 (former)
• Wyoming Highway 220