A Spot-tag is a 12-amino acid peptide tag recognized by a single-domain antibody (sdAb, or nanobody). Due to the small size of a Spot-tag (12 amino acids) and the robust Spot-nanobody (14.7 kD) that specifically binds to Spot-tagged proteins, Spot-tag can be used for multiple capture and detection applications: Immunoprecipitation, affinity purification, immunofluorescence, and super-resolution microscopy.[1] Recombinant proteins can be engineered to express the Spot-tag.
Spot-tag Sequence
Amino acid sequence
PDRVRAVSHWSS
Codon optimized DNA sequence
| Human | CCA GAC CGC GTG CGC GCC GTG AGC CAT TGG AGC AGC |
| S. cerevisiae | CCA GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT |
| E. coli | CCG GAT CGC GTG CGC GCA GTC TCT CAC TGG AGC AGC |
See also
References
- ↑ Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Pisak-Lukáts B, Endesfelder U, Rothbauer U (2018). "A peptide tag-specific nanobody enables high-quality labeling for STORM imaging". Nature Communications. 9 (1): 930. Bibcode:2018NatCo...9..930V. doi:10.1038/s41467-018-03191-2. PMC 5834503. PMID 29500346.
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